|Engineering Escherichia coli for the production of proteins
|Date Posted||Friday, 12 October 2012|
Dept. of Biochemistry and Biophysics
|Job Description||The bacterium Escherichia coli is the most widely used vehicle for the production of proteins. So far, our laboratory has focused on the production of membrane proteins in E. coli. Using a variety of different techniques we have been able to identify the bottlenecks hampering the production of membrane proteins. This information was subsequently used to engineer strains with improved membrane protein production characteristics. Now we aim at identifying the bottlenecks hampering the production of proteins in the cytoplasm and periplasm of E. coli. This information will be used to engineer strains for the production of these proteins, similar as to what we have done for membrane proteins. We use a host of different techniques in our research; e.g., genetics, molecular biology, flow cytometry, fluorescence microscopy, different kinds of gel-electrophoresis techniques, mass spectrometry and whole genome sequencing. Our laboratory works in close collaboration with the laboratory of Dr. Joen Luirink (http://www.bio.vu.nl/vakgroepen/molmic/) and Xbrane Bioscience AB (www.xbrane.com).
1. Wagner et al. Mol Cell Proteomics. 2007, 6:1527-50.
2. Wagner et al. Proc Natl Acad Sci U S A. 2008, 38:14371-6.
3. Wickström et al. J Mol Biol 2011, 409:124-35.
4. Luirink et al. Biochim Biopys Acta 2012, 1817(6):965-76.
5. Schlegel et al. J Mol Biol 2012, http://dx.doi.org/10.1016/j.jmb.2012.07.019
|Qualification||Background in molecular biology/biochemistry/microbiology/bioengineering.|
|How to Apply||Send an Email to: firstname.lastname@example.org. Include a short motivation, a CV (plus diplomas and certificates/date when you expect to finish your studies), and the contact details of at least 2 references in your application.|
|Supervisor/ Principal Investigator||Jan-Willem de Gier|
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